Download or read book SILVER AND GOLD BASED AUTOMETALLOGRAPHY OF NANOGOLD. written by and published by . This book was released on 2002 with total page 19 pages. Available in PDF, EPUB and Kindle. Book excerpt: For many applications, silver salt-based autometallography (often also called silver enhancement or silver development) is required to visualize colloidal gold (1-5 nm in diameter) or the small 1.4 nm Nanogold{reg_sign} particles (Nanoprobes, Yaphank, NY, USA). Although even Nanogold may be seen directly by scanning-transmission electron microscopy (STEM), by transmission EM (TEM; in thin sections without stain or ice-embedded cryo-EM samples), energy filtered TEM, and scanning EM (SEM), silver enhancement makes viewing in the EM more facile since the particles are enlarged to approximately 10 to 20 nm, convenient for most specimens. Autometallographic (AMG) enhancement is required in order to visualize smaller gold particles such as Nanogold for light microscopy (LM) or in blots or gels. This chapter includes the following protocols: Protocol for HQ silver enhancement of Nanogold; Protocols for use of silver-enhanced Nanogold with osmium tetroxide--(A) Procedure using reduced concentration of OsO4; (B) Procedures for gold toning; Protocol for HQ silver enhancement of Nanogold in pre-embedding immunocytochemistry for cell cultures; Protocol for gold enhancement of Nanogold for EM; Protocol for gold enhancement of Nanogold for LM; Protocol for staining blots with Nanogold and silver enhancement; and Protocol for staining gels with Nanogold and silver enhancement.