A Practical Guide to Protein and Peptide Purification for Microsequencing

A Practical Guide to Protein and Peptide Purification for Microsequencing
Author :
Publisher : Elsevier
Total Pages : 205
Release :
ISBN-10 : 9780080924618
ISBN-13 : 0080924611
Rating : 4/5 (611 Downloads)

Book Synopsis A Practical Guide to Protein and Peptide Purification for Microsequencing by : Paul T. Matsudaira

Download or read book A Practical Guide to Protein and Peptide Purification for Microsequencing written by Paul T. Matsudaira and published by Elsevier. This book was released on 2012-12-02 with total page 205 pages. Available in PDF, EPUB and Kindle. Book excerpt: Why a Second Edition?The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry. - Mass spectrophotometric characterization of proteins and peptides - N-terminal sequencing of proteins with blocked N termini - Internal amino acid sequence analysis after protease digestion in-gel and on-blot - Improved microscale peptide purification methods - Computer analysis of protein sequences - New protocols tested and refined through everyday use in authors' laboratories - Updated reference chapter covering all aspects of protein microsequencing

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