Download or read book Root-associated Fungal Diversity, Nutrient Mineralization and Micro-climatic Interconnections written by Korena Mafune and published by . This book was released on 2021 with total page 183 pages. Available in PDF, EPUB and Kindle. Book excerpt: The temperate rainforests of western Washington are commonly recognized for their extensive areas of old-growth forest and more recently their unique canopy soil environment. Over the last 40 years, fundamental studies on canopy soils have emphasized their importance as structural and functional components of these ecosystems; but there remain many unknowns on the biotic and abiotic processes in canopy soil environments, how these may be impacted by climate change, and the implications this may have on host tree resiliency. For example, old-growth bigleaf maple trees (Acer macrophyllum Pursh.) grow extensive adventitious roots that form fungal associations. However, no studies have explored the diversity of these adventitious canopy root associated fungal communities and how they compare to forest floor rooting networks. Further, no studies have explored the seasonal mineralization rates of plant available nutrients in canopy soils and how they enhance forest-level nutrient cycling. Therefore, this study aimed to not only compare these biotic and abiotic processes between the two soil environments, but it also aimed to shed light on how these processes may be impacted by increased and decreased rainfall amounts to better understand how these trees may be affected by climate change. Prior to identifying fungal communities associating with roots in canopy and forest floor soil environments, a methodological approach for long-read sequencing of fungi was designed and tested on the MinION Nanopore Sequencer. To assess the capabilities of the MinION, three fungal mock communities were sequenced. Each had varying ratios of 16 taxa. The MinION recovered all mock community members, when mixed at equal ratios. Highly accurate consensus sequences were derived and identified to species level, proving that the MinION was suitable as a practical alternative to gain insights on root-associated fungal communities. After benchmarking this technology, roots were collected from canopy and forest floor environments to determine if there were any differences in the percent of fungal colonization. There was no significant difference between the percent of fungi colonizing adventitious canopy roots (56.5% ± 5.4) and forest floor roots (65.1% ± 3.6). Subsequently, a rainfall experiment was implemented and root associated fungal communities were identified seasonally (excluding winter) over the duration of one year. At ambient conditions, root associated fungal community composition was significantly different between the two old-growth sites and also between canopy and forest floor environments. However, these communities did not shift in response to seasonal changes. In canopy soil environments, the increased and decreased rainfall experiments and site differences also significantly affected fungal community composition; seasonality also had an effect. The MinION was able to identify a diversity of obligate mutualists and facultative endophytes. There were several species associating with adventitious canopy roots that have never been reported to associate with bigleaf maple prior to this study. Nitrogen (N) and phosphorus (P) mineralization rates were also determined seasonally during the rainfall experiment as well as annual N and P pools. In canopy soil environments, both the rainfall treatments and seasonality had a significant effect on N mineralization rates. Phosphorus mineralization rates were also impacted by the rainfall treatments. On a per mass basis at ambient conditions, canopy soils have higher rates of net N (355.3 ± 54.7 mg N kg-1 yr-1) and net P mineralization (387.6 ± 34.5 mg PO4-P kg-1 yr-1) than forest floor soils (58.2 ± 3.9 mg N kg-1 yr-1 and 387.6 ± 34.5 mg PO4-P kg-1 yr-1). When converted to an areal basis, canopy soils enhanced the annual NO3-N, NH4-N, and PO4-P mineralization pools by 5.2%, 48.4%, and 3.7%, respectively. Additionally, some of these resources are leaching to the forest floor soil environment. The first part of this study benchmarked a methodological approach that was utilized throughout the project and allowed the inference of genus and species-level resolution in canopy and forest floor environments. The other two parts of this study demonstrated that canopy soils provide an extra compartment for nutrients and that adventitious rooting systems are associating with a diversity of fungi distinct from forest floor environments. Further, higher and lower inputs of rainfall impact these biotic interactions, as well as the nutrient dynamics. Collectively, this research reveals that fungal communities associating with adventitious roots may be acting as adaptive facilitators to environmental extremes (e.g., climatic changes) and that biogeochemical cycles in canopy soils and their inputs to the ecosystem should not be overlooked.