The Effective Temperatures of Gas-phase Biological Molecules Stored in a Quadrupole Ion Trap
Author | : Geng Li |
Publisher | : |
Total Pages | : 164 |
Release | : 2008 |
ISBN-10 | : 0494388692 |
ISBN-13 | : 9780494388693 |
Rating | : 4/5 (693 Downloads) |
Download or read book The Effective Temperatures of Gas-phase Biological Molecules Stored in a Quadrupole Ion Trap written by Geng Li and published by . This book was released on 2008 with total page 164 pages. Available in PDF, EPUB and Kindle. Book excerpt: The internal energy distribution undergoing collision-induced dissociation (CID) in the cell of a quadrupole ion trap (QIT) mass spectrometer can be characterized by the effective temperature, which would help determine the activation energy and entropy. Here, the relationship in a Bruker QIT has been reexamined based on kinetic measurements at two qz values. An observed linear relationship between ion effective temperatures and resonant activation amplitudes appears qualitatively different from simplified theoretical simulation, which is in agreement with previous results on Finnigan ion trap. However, the normalization process aiming to compensate for the m/z dependency of the required fragmentation energy that is used successfully in Finnigan-geometry traps is not entirely successful for use in Bruker-geometry trans. The calibration determined here roughly indicates the relationship between ion effective temperature, resonant excitation amplitude and ion m/z. However, Successful extraction of the dissociation energetics still requires a better accuracy of the calibration.